Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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jove.com
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
36.6K Views. Yale University School of Medicine. Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
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44 reactions · 14 shares | Zebrafish grow at an extremely fast rate, developing as much in a day as a human embryo develops in one month. For context, a fertilized zebrafish egg develops and hatches within 48 hours. https://bit.ly/3HjqU1A The first 22 hours of zebrafish development (blood vessels shown in green) Daniel Castranova Dr. Brant M. Weinstein National Institutes of Health (NIH) NICHD Bethesda, Maryland, USA Confocal 4X (Objective Lens Magnification) | Nikon Small World | Facebook
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