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Four membrane proteins are represented in the figure below. Boxes represent membrane-spanning sequences and arrows mark sites for cleavage by signal peptidase. Plusses and minuses represent charged amino acids at the ends of some transmembrane segments. Predict how each of the mature proteins will be arranged across the membrane of the ER. Indicate the N and C termini relative to the cytosol and the lumen of the ER. Explain your reasoning for each.
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